We observed CYK-1::GFP localized in the germline similar to endogenous CYK-1 (Severson, Baillie, & Bowerman, 2002; Figure S8a). BWM appeared grossly normal in these animals, and we did not observe CYK-1::GFP at any structure in BWM, including DBs.
As reported previously, the promoters of the a, b, and f isoforms drove expression in the whole body except for the pharynx (isoform a), a few tissues (isoform b), and almost all tissues except the gonad (isoform f).We also confirmed Venus expression in head neurons including ASER, by these promoters.
This operon, when introduced as a transgene, fully restored bacterial adhesion and tail swelling to infected animals, and exhibited the same expression in cells K, F, and U, with a diffuse cytoplasmic distribution of the GFP reporter.
mhIs9 males contain a lin-17::gfp construct that was expressed in the membranes of the T, B cells and their descendants as well as the F, P11, P12 and vuval precursor cells.
FHOD-1::GFP is localized in F-actin rich muscles, including BWMs, pharyngeal muscles, and vulval muscles. FHOD-1 is present diffusely in the elongated bands of F-actin rich embryonic myoblasts. FHOD-1 is not detectable in F-actin rich BWMs of L1 larvae but appears as puncta along muscle edges and as striations across the BWM contractile lattice in L2 and L3 larvae.
Transgenic animals carrying this reporter showed strong fluorescence in the presumptive rectal epithelial cells K, F, and U. This localization was confirmed using a hindgut-specific marker, lin-48p::GFP, which expresses in the same cells.