Expr105

This pattern is first expressed as a few nuclei in 3-fold embryos. A few nuclei also stain in young larvae. In adults expression is evident posteriorly to the vulva. This expression is reproducible but seems to be on the threshhold of observability as sometimes expression is not seen (due to less efficient staining?) It appears to stain the A and B epithelial cells which connect the uterus to the hypodermal syncytium, ie in the hypodermal ridge posterior to the vulva. We have seen one example (due to the low threshhold?) where the posterior third of the anterior component of the A and B epithelial cells, as well as the posterior component stain. This does seem to follow with the possibility of some concentration gradient evident in the gene product produced. There is occasionally a second component. This is seen in the larval stages and is anterior, and more frequently posterior, intestinal nuclei. In adults some diffuse intestinal staining is also seen.

Expr112

This neuronal pattern gives expression throughout all life stages of C. elegans. Staining is first seen in precomma stage embryos. In 3-fold embryos expression appears to be localised in nuclei around the pharynx and tail. In young larvae there is extensive staining in all of the head ganglia and the anal ganglion. The ventral nerve cord and its cell bodies also show strong expression. As the worm ages the expression in the head ganglia is reduced to a number of nuclei in the ventral (ie AIML/R?) and lateral ganglion, although expression in the nerve ring and the ventral nerve cord is still observed. There appears to be some mosaicism in the expression as some larvae show stronger staining in the ventral nerve cord and more nuclei stain in the head ganglia, whereas in other larvae head expression is reduced and only the cell bodies of the ventral nerve cord stain. Diffuse expression is sometimes observed in the metacorpus and terminal bulb of the pharynx in larvae and adults.