FHOD-1::GFP is localized in F-actin rich muscles, including BWMs, pharyngeal muscles, and vulval muscles. FHOD-1 is present diffusely in the elongated bands of F-actin rich embryonic myoblasts. FHOD-1 is not detectable in F-actin rich BWMs of L1 larvae but appears as puncta along muscle edges and as striations across the BWM contractile lattice in L2 and L3 larvae.
Immunoreactivity was concentrated in nervous system regions rich in synapses, including the nerve ring, the dorsal nerve cord and the ventral nerve cord.
The antibody specifically stained the synapse-rich nerve ring, ventral nerve cord and dorsal nerve cord in wild-type but not in unc-43(n1186) or unc-43(e755) animals.
Endogenous DVC1 was predominantly expressed during S and G2 phases of the cell cycle. Upon exit from mitosis, DVC1 expression was rapidly downregulated with kinetics similar to that of known APC-Cdh1 targets such as cyclin A, and reaccumulated as cells entered S phase.