DRAG-1::GFP was present in pharyngeal, hypodermal and intestinal cells. Robust expression in the M lineage was also detected from the 1-M to 4-M stage. DRAG-1::GFP signal was reduced (40%, n=20) or undetectable (60%, n=20) at the 8-M stage, and completely undetectable beyond the 8-M stage.
UNC-130::GFP expression is not detectable in the early M lineage from the 1-M to the 4-M stage. Beginning at the 8-M stage and continuing through the 18-M stage, UNC-130::GFP expression is visible in all the ventral, but not in any of the dorsal, M lineage cells. It was noticed that M.vlp and M.vrp and their descendants exhibited relatively higher levels of UNC-130::GFP expression than their anterior counterparts.
UNC-130::GFP expression is not detectable in the early M lineage from the 1-M to the 4-M stage. Beginning at the 8-M stage and continuing through the 18-M stage, UNC-130::GFP expression is visible in all the ventral, but not in any of the dorsal, M lineage cells. It was noticed that M.vlp and M.vrp and their descendants exhibited relatively higher levels of UNC-130::GFP expression than their anterior counterparts.