Expr4390

Confocal microscopy indicated that the aman-2 promoter is most active in the gut wall, pharynx and grinder, hypodermal cells, and cells of the nervous system (ventral nerve cord cells, neurons surrounding the pharyngeal bulb and in the head) in adult animals; also rather ubiquitous expression was seen in larvae.

Expr13642

A MEC-19:: GFP translational fusion was found throughout the TRN neurite and also on the plasma membrane and spots within the TRN cell body; its expression overlapped only partially with MEC-4 and MEC-2 (Topalidou and Chalfie 2011) in the proximal neurite and cell body. In the cell body, MEC-19 spots also were found to partially overlap with the Golgi marker AMAN-2::TagRFP.

Expr12257

During embryogenesis, at the pre-comma stage, the GFP signal was first observed in the RID precursor (ABalappaap) before its final round of cell division. After the division, GFP signals were observed in both progenies. After the RID sister cell had undergone programmed cell death, the RID terminal differentiation marker Pceh-10::wCherry was observed in the surviving cell. Coexpression of the ceh-10 and unc-3 reporters in the RID neuron persisted into the larval stage. Similar to what has been reported previously (Prasad et al., 1998, 2008; Kratsios et al., 2012), the unc-3 fosmid reporter also exhibited expression in cholinergic motor neurons.