Expr13290

vang-1 is expressed in VNC neurons. VANG-1 (n = 9) was observed to localize to cell membranes and is enriched at contracting cell contacts. In addition, VANG-1 is localized at rosette foci. These localization dynamics resemble that of endogenous myosin II (NMY-2) (Dickinson et al., 2013), which is also found on contracting cell contacts and rosette foci. VANG-1 exhibited approximately 4-fold enrichment at contracting edges versus non-contracting edges and approximately 7-fold enrichment at the rosette center relative to the planar contacts between cells engaged in the rosette.

Expr15034

To study UNC-119 protein localization, we generated a C-terminal GFP knock-in line using the SEC-approach (Dickinson et al., 2015) and observed GFP expression in all neurons, as previously reported (Lim et al., 1999; Maduro and Pilgrim, 1995). The GFP signal can be detected in axons (ventral nerve cord and nerve ring) as well as dendrites (amphid nerve). Strikingly, the protein is not enriched in the cell bodies, which suggests that the protein is not freely diffusing in the cytoplasm. Although expression is mainly neuronal, low levels are also present in the hypodermal seam cells.