Expr2624

Promoter-GFP transgenes indicated that lam-3 is expressed during gastrulation and through embryogenesis in pharyngeal, intestinal and epidermal cells. In the larvae, lam-3 gene expression is maintained in the spermatheca and in the pharyngeal m3-m8 and mc cells.

Expr10827

LAM-3/Laminin alpha-A immunostaining was evident between the germ layers, including on most surfaces of the endoderm. In particular, LAM-3 localized around the surfaces of the endoderm row 8 cells, just before and as the somatic gonad precursors (SGPs) extended projections between the anterior surfaces of these endoderm cells and the posterior surfaces of the primordial germ cells (PGCs). A functional LAM-1-GFP fusion protein showed a pattern of localization similar to LAM-3, including around the gonadal primordium at later stages. Therefore, laminin basement membrane is present on endodermal surfaces where the SGPs migrate and extend projections around the PGCs.

Expr2621

lam-3 is first detected between tissue layers near the end of gastrulation and then becomes localized along the muscle cells as the embryo begins to elongate. By the 3-fold stage of elongation, staining along the muscle quadrants is weaker and becomes restricted to a band at the center of each quadrant, which colocalizes with the dorsal and ventral sublateral nerve tracts in the adult. Staining is intense around the pharynx, pharyngeal-intestinal valve, and intestine during morphogenesis. In larvae and adults, the antiserum stains the spermatheca strongly and only weakly stains the pharynx, the intestine and the excretory canal.