Expression of gcy-19 was reported to be weak in both of ASE neurons (Ortiz et al., 2006), but here was observed ASER-biased expression in four independent transgenic lines.
Using immunofluorescence, we found that CEC-1 was nuclear and broadly expressed in the embryo, larval and adult animals. Although CEC-1 was suggested to be soma-specific (AGOSTONI et al. 1996), its transcript was detected in the oogenic germline by RNA-Seq (ORTIZ et al. 2014; BOECK et al. 2016). Consistent with these findings, while we do not observe CEC-1 in the distal germline, it is detected in proximal germ cells and oocytes, albeit at a lower level than somatic cells.
In the meiotic cells of the gonad including oocytes and in the interphase cells of 200-cell embryos, DNA topoisomerase I was localized in nuclei. At the comma stage, a more narrowed concentration of DNA topoisomerase I was observed in the nucleoli of gut cells.
GFP was shown to express in the excretory cells, coelomocytes, head neurons, hypodermal cells, and in the pharynx of C. elegans. This expression pattern was further confirmed by antibody staining using polyclonal antibodies against UCH/CeUBP130. Excretory cells were identified by the 'I-i' shape GFP expression in the head region). GFP was also shown to express in coelomocytes.