Pins-7::gfp is expressed in multiple tissues, including several head neurons and the intestine, consistent with previous findings (Murphy et al., 2007). The ins-7-expressing head neurons include ADF, ASI, ASK, and URX among others.
As previously reported, pitp-1 is expressed in many neurons. We observed a co-lo- calization of the GFP and mCherry fluorescence in the AQR, PQR, and URX neurons, as well as in the BAG O2-sensing neurons (the BAG neurons act reciprocally to the URX neurons and are activated at low O2 levels), indicating that pitp-1 is co-expressed with glb-5 in O2-sensing neurons.