The abts-2 promoter expressed GFP in a single neuron in the tail ganglion. This pattern may indicate remarkable specificity but also may represent inappropriate expression of an incomplete gene.
The pfn-3 promoter::GFP fusion construct yielded no expression, suggesting that an additional enhancer element may be required for its expression or an inhibitory cis-element may be contained in the construct.
ceh-19aprom::gfp fusion: no GFP expression was observed, throughout the normal life cycle or in the dauer, suggesting the ceh-19a transcript may not be functional or may only be expressed in circumstances not assayed.
GFP expression was observed from the comma stage embryo. The GFP may be in the P cell lineages from late embryogenesis and through the L1 stage but this seems to disappear at the L2 stage. A few cells in the body wall expressed GFP in older larvae and adults; this may be muscle but was very weak and may be hypodermis in the head. There may also be GFP expression in vulval and rectal muscle in the L4 and young adult. No intestinal expression of GFP was detected.
ACBP-6 is uniquely expressed in specific head, body and tail neurons. The neuronal expression of pacbp- 6::GFP in the mid-body may be the CAN neurons, whereas the neuron in the head is located dorsally posterior to the posterior bulb of the pharynx and may be the AVG command interneuron.