sem-4 is expressed in all rectal cells in L1s hermaphrodites (i.e., Y, U, B, F, K, and K') and in all rectal cells, including P12.pa, in L3 or older worms.
The Pmir-83::GFP reporter was widely expressed at all stages of development and across a range of tissues such as the intestine, neurons, and body wall muscles. Furthermore, we observed a significant decrease in the expression of miR- 83 between the L3 and L4 stages, which is consistent with a previous report (Martinez et al., 2008a,b), and a further decrease from day 1 of the adult stage through day 12. About 44% decrease in GFP expression driven by miR-83 was observed in older adults (day 12) compared to young adults (day 4).
Pdaf-6GFP was expressed in the amphid sheath glia. Expression was also seen in amphid socket cells, the phasmid sensory organ sheath and socket cells, cells of the excretory system (the excretory canal, duct, pore, and gland cells), the vulval E and F cells, the K, K', F, and U rectal epithelial cells, and less frequently in posterior intestinal cells.
About 20% GABAergic presynaptic terminals (RFP puncta) co-localized with postsynaptic NKB-1 (GFP) localization (103 co-localization with GFP puncta in totally observed 470 RFP puncta (n = 20 animals)). The majority of the postsynaptic Na+/K+ ATPase localization did not overlap with that of GABAergic synapses, suggesting a predominant localization of the Na+/K+ ATPase at cholinergic postsynaptic sites.