Expr9045

Using antibodies against MOG-3 authors found one major 53 kD protein in extracts from wild type and masculinized fem-3(gf) animals, but not in mog-3 mutants. MOG-3 was more abundant in wild type than in fem-3(gf) animals indicating that the protein is expressed in embryos or oocytes, which are absent in the masculinized fem-3(gf) mutant.

Expr13433

fmo-4::gf pwas expressed strongly in hypodermal cells, excluding the seam cells and vulval cells, consistent with previous studies. We also observed fmo-4::gfp expression in cells in the ventral nerve cord.

Expr13404

cdc-25.3 mRNA is highly expressed in N2 hermaphrodites at the young-adult stage, when oogenesis actively occurs, and in feminized fem-1(lf) hermaphrodites, but not in adult males or in masculinized fem-3(gf) hermaphrodites. These results indicate that cdc-25.3 is preferentially expressed during oogenesis.

Expr3675

Compared to wild-type expression, ccf-1 is present at a higher level (148%) in fem-1(lf) adults that produce oocytes but no sperm. ccf-1 expression is reduced in fem-3(gf) (52%) and glp-4(lf) (31%) which produce sperm but no oocytes and have a severely reduced and undifferentiated germline, respectively. The higher expression of ccf-1 in fem-3(gf) than in glp-4 adults could suggest a weak expression of ccf-1 in the male germline. Altogether these results suggest that ccf-1, although present at all stages, is expressed at a higher level in embryos and adults, in which expression is dependant upon the germ line.

Expr10041

In situ hybridizations of dissected gonads were performed to ascertain the precise spatial pattern of spe-44 expression. The fem- 3(gf) and fem-1(lf) mutations were used to restrict hermaphrodite gametogenesis to sperm or oocyte production, respectively. Abundant expression was first detected in fem-3(gf) animals during the L3 stage in the early meiotic germline. DAPI staining of the chromatin indicated that expression coincides with early pachytene of meiosis I. By contrast, spe-44 expression was undetectable in fem-1(lf) germlines at any stage of development. Thus, spe-44 expression is restricted to the sperm-producing germline at a time and place consistent with the regulation of sperm gene expression.

Expr1941

spe-6 mRNA could be amplified from fem-3(q23gf) hermaphrodite mRNA (this mutant has hermphrodite soma but makes only sperm germline), but not not from fem-1(hc17ts) hermaphrodite mRNA (mutant hermaphrodites make only oocytes, no sperm); cDNA clone ca01c03 (GenBank accesion nos. AW057333 and AW057136) are from spermatogenesis-specific libraries; SPE-6 protein only detected in extracts from sperm-producing worms (i.e. fem-3(gf), not fem-1(lf) on western blots.

Expr2264

This construct was expressed in several tissues: AC, spermatheca and K lineage. In all cases expression could be detected at the appropriate time. In addition, unexpected vulval expression was found from the early to mid fourth larval (L4) stage in the vulF cells, which are the dorsal-most 1 vulval progeny. The expression appeared to be 1-vulva-specific because the vulval lin-3::lacZ expression was absent in animals bearing a strong lin-12(gf) mutation, which confers a multivulva phenotype consisting of only 2 lineages.

Expr9997

spe-5 mRNA is dramatically up regulated in wild type (wt) males as compared to spe-5 mutant males and, also, in fem-3 gain of function (gf; female soma, male germline) as compared to fem-3 loss of function (lf; female soma, female germline) worms. Adult wt hermaphrodites continue to show significant spe-5 expression after they have completed spermatogenesis, as do fem-3 loss of function (lf; female soma, female germline) worms. These data indicate that, while spe-5 expression is up regulated during spermatogenesis, it is also expressed in one or more other tissues.