Expr1508
In L1, after induction by 0.1 mM CdCl2, the reporter enzyme was markedly induced in the 20 cells constitute the intestine. While in adults, the Cadmium induced only a low level of beta-gal activity in the intestinal cells. Expression of lacZ in pharyngeal cells was not significantly affected by Cadmium or development stage.
Expr14247
The Pmir-83::GFP reporter was widely expressed at all stages of development and across a range of tissues such as the intestine, neurons, and body wall muscles. Furthermore, we observed a significant decrease in the expression of miR- 83 between the L3 and L4 stages, which is consistent with a previous report (Martinez et al., 2008a,b), and a further decrease from day 1 of the adult stage through day 12. About 44% decrease in GFP expression driven by miR-83 was observed in older adults (day 12) compared to young adults (day 4).