The Pmir-83::GFP reporter was widely expressed at all stages of development and across a range of tissues such as the intestine, neurons, and body wall muscles. Furthermore, we observed a significant decrease in the expression of miR- 83 between the L3 and L4 stages, which is consistent with a previous report (Martinez et al., 2008a,b), and a further decrease from day 1 of the adult stage through day 12. About 44% decrease in GFP expression driven by miR-83 was observed in older adults (day 12) compared to young adults (day 4).
Expression in the alimentary canal: Strong and consistent expression in pm2, pm4, pm5, mc1, mc2. Weak or rare expression in pm3, pm6, pm7, pm8, K.a/K$(B!G(B cells.
We noted ciliary localization of the SRG-36 G protein-coupled receptor to the ASI cilia but also detected significant accumulation at the periciliary membrane compartment (PCMC) in wild-type animals.
We noted ciliary localization of the SRBC-64 G protein-coupled receptor to the ASK cilia but also detected significant accumulation at the periciliary membrane compartment (PCMC) in wild-type animals.
Weak diffuse expression is seen around the metacorpus of the pharynx of larvae and adults, and may correspond to neuronal staining. This gene is a member of the ODR-10 family of G-protein coupled receptors.
CATP-6::GFP is expressed in multiple tissues throughout development. These include a) many neurons in the head and tail, b) all body muscles, c) most pharyngeal cells, particularly in the posterior bulb, d) vulval muscles, e) coelomocytes, f) spermatheca, g) gonadal sheath cells, and h) lateral hypodermis.
A G-protein coupled receptor homologue, this gene has a restricted expression pattern in ADEsoL/R and PDEsoL/R in late larval stages. These are the socket cells of the deirid and post-deirid sensilla respectively, specialised nerve-accessory cells which form a toroid around the sensory process of the sensillum and act as an interface between it and the hypodermis