Expr16434

Imaging by spinning-disc confocal microscopy revealed that CAP-1 is expressed in multiple adult tissues, including the germline, spermatheca, pharynx and vulva. It was also found to localize at the apical side of gut epithelial cells, in striations along body-wall muscle cells and in the intestinal-rectal valve. We found CAP-1 to be expressed in all germ cells, where it localized to cell cortices and was highly enriched at rachis bridges and within the actomyosin corset surrounding the rachis . CAP-1 was also observed surrounding the germ cell nuclei. CAP-1 colocalized with PLST-1 and with NMY-2 along the rachis, confirming that it is an integral component of the actomyosin corset in the syncytial germline.