Expr14294

Long Isoform. The reporter for long VAB-3 isoforms that include the Paired homology domain was not expressed in BAG neurons.

Expr16018

The extra-long MEC-2E::RFP is expressed specifically in touch neurons. Similar to the long MEC-2A isoform, the extra-long MEC-2E isoform is expressed in a punctate pattern in both the cell bodies and the neurites of the touch neurons, and this expression is abolished in mec-8 mutants. Unlike the short MEC-2B isoform, there is no detectable expression of extra-long MEC-2E in head neurons.

Expr13149

The sms-1 long isoform was expressed in the pharyngeal gland cells, spermacecae, and coelomocytes.

Expr10609

ZFP-1::GFP was expressed in somatic cells at all developmental stages of C. elegans. High expression in oocytes and lower expression in the distal germline was observed.Immunostaining showed high expression in maturing oocyte nuclei and lower expression in the rest of the germline. The long isoform of the protein is expressed in the germline, while the short isoform is not. The immunostaining results are consistent with the expression patterns of the short and long isoforms of ZFP-1 determined by western, where the long isoform is predominantly expressed in embryos and in adult worms with developed germline tissue and the short isoform more abundant at the larval stages. The authors conclude that only the long ZFP-1 isoform is expressed in the germline and localizes to chromosomes there.

Expr11845

At the L1 larval stage, MADD-4L-GFP is mainly localized at the nerve ring and at the dorsal cord. MADD-4L refers to long isoforms (which globally refers to MADD-4A andMADD-4C).

Expr16017

Tagging the canonical long mec-2A isoform with RFP revealed strong expression in the touch neurons, with puncta visible in both the cell body and throughout the neurite, in accordance with previous imaging using antibody staining.

Expr1582

CNR8 showed greatest expression in L3, weak bands were also detected in L4 and adults after long time exposure. Only a single band of 3.1 kb was detected, except in L4 stage a 2.1 kb band is visible.

Expr14292

Short isoform. Both the pan-VAB-3 reporter transgene and the reporter for short homeodomain-only VAB-3 isoforms were expressed in BAG neurons. By contrast, the reporter for long VAB-3 isoforms that include the Paired homology domain was not expressed in BAG neurons.

Expr8356

The long TEN-1 isoform is expressed in the developing and adult pharynx. The GFP::TEN-1 transgene is expressed in the developing pharynx of the early embryo and outlines the adult pharynx. Expression of the kdEx121 is also found in some head neurons.

Expr16535

Long isoform. We generated strains that express either long(ex7+) or short(ex7-) MBL-1 protein isoforms, tagged with mCherry, under both endogenous promoters together, and investigated their subcellular localization at the L4 larval stage (when most tissue development is complete). Expression of MBL-1 was detected in various tissues in a pattern consistent with previous studies [38, 39], specifically in the head and tail ganglia, the ventral nerve cord (VNC), the spermatheca, and the posterior gut. Consistent with the human longer MBNL isoform, mCherry signals showed a distinct nuclear localization when linked with long(ex7+) isoforms, most noticeably in, but not limited to, cells of the gut and the head region. In contrast, short(ex7-) isoforms showed a more diffuse pattern, consistent with localization both in the nucleus and cytoplasm. We note that the expression in the gut might be ectopic and a possible consequence of the presence of the unc-54 30-UTR, as reported before [43].