The egl-27 reporter is expressed in the Y cell in the 1.5-fold embryonic stage as well as during the L1 larval stage, which precedes the initiation of Y transdifferentiation.
Consistent with previous findings (Kim and Li, 2004), we found that flp-20 is expressed in the TRNs (ALM, AVM, PLM, PVM), along with a few other neurons (ASE, LUA, and PVC).
The expression pattern of Pncr-2/GFP/49.26 differs from what Li et al. (2004) report in that expression in the gonadal sheath was not observed but was observed in the ventral nerve cord.