Expr14687

Consistent with previous findings (Kim and Li, 2004), we found that flp-20 is expressed in the TRNs (ALM, AVM, PLM, PVM), along with a few other neurons (ASE, LUA, and PVC).

Expr12628

The expression pattern of Pncr-2/GFP/49.26 differs from what Li et al. (2004) report in that expression in the gonadal sheath was not observed but was observed in the ventral nerve cord.

Expr14346

We inserted an in-frame gfp cassette into the endogenous tmc-1 locus using the CRISPR/Cas9 knockin method (Li et al., 2015). Using this approach, we observed TMC-1 expression in vulval muscles as well as several head neurons and body wall muscles.

Expr13409

When the fluorescent reporter Venus was fused to the 3.5 kb lury-1 promoter (lury-1prom), the expression of Venus was observed only in two classes of pharyngeal neurons. These neurons were identified as the M1 and M2 neurons by their morphology, position, and the co-expression of established cell markers (Kim and Li, 2004; Refai et al., 2013). We found that the lury-1prom::Venus reporter was robustly expressed in M1 in all larval stages and in adults, whereas its expression in M2 was not detectable in most L1-L3 worms but started to increase from the L4 stage.

Expr11266

In wild-type worms, in addition to being in the amphid neurons and the gut, the protein is also found in coelomocytes. In the coelomocytes,the full-length DAF-28::GFP protein was present in vacuoles that have been shown to take up the endocytosis marker FM4-64 (Fares and Greenwald, 2001). daf-28::gfp transcriptional reporters are not expressed in coelomocytes (Li et al., 2003) (G.K., S.T., and P.N., unpublished data). The presence of DAF-28::GFP in coelomocytes,therefore, is the result of uptake of the protein from the psuedocoelomic fluid rather than that of transcription of daf-28 in these cells.