Consistent with previous findings (Kim and Li, 2004), we found that flp-20 is expressed in the TRNs (ALM, AVM, PLM, PVM), along with a few other neurons (ASE, LUA, and PVC).
The expression pattern of Pncr-2/GFP/49.26 differs from what Li et al. (2004) report in that expression in the gonadal sheath was not observed but was observed in the ventral nerve cord.
We inserted an in-frame gfp cassette into the endogenous tmc-1 locus using the CRISPR/Cas9 knockin method (Li et al., 2015). Using this approach, we observed TMC-1 expression in vulval muscles as well as several head neurons and body wall muscles.
daf-18 message was present predominantly in the germline in larvae and adults (93%, n = 88). This is a specific signal not detected in the daf-18 deletion mutant (n = 89) or in glp-4(bn2) animals (n = 112), where little germline proliferation occurs.
DRAG-1::GFP was present in pharyngeal, hypodermal and intestinal cells. Robust expression in the M lineage was also detected from the 1-M to 4-M stage. DRAG-1::GFP signal was reduced (40%, n=20) or undetectable (60%, n=20) at the 8-M stage, and completely undetectable beyond the 8-M stage.