Consistent with previous findings (Kim and Li, 2004), we found that flp-20 is expressed in the TRNs (ALM, AVM, PLM, PVM), along with a few other neurons (ASE, LUA, and PVC).
The expression pattern of Pncr-2/GFP/49.26 differs from what Li et al. (2004) report in that expression in the gonadal sheath was not observed but was observed in the ventral nerve cord.
We inserted an in-frame gfp cassette into the endogenous tmc-1 locus using the CRISPR/Cas9 knockin method (Li et al., 2015). Using this approach, we observed TMC-1 expression in vulval muscles as well as several head neurons and body wall muscles.
DAF-19A/B were expressed only in nonciliated neurons and not in ciliated sensory neurons. In summary, DAF-19A/B are expressed in 200 to 240 nonciliated neurons.
Expression in the alimentary canal: Strong and consistent expression in pm2, pm4, pm5, mc1, mc2. Weak or rare expression in pm3, pm6, pm7, pm8, K.a/K$(B!G(B cells.
The B+B enhancer (pOK17.13) activates frequent expression only in pharyngeal muscles m3, m4, m5 and m7, with occasional expression in m1. No expression has been observed in m6 or m2.