Expr15037

We found that adult animals fed a normal laboratory diet of E. coli HB101 express cysl-1 predominately in neurons with faint expression in hypodermal cells.

Expr3046

Transgenic expression of either GFP::GLR-2(Q) or GFP::GLR-2(R) in both the wild-type laboratory strain N2 and in a glr-2 deletion strain resulted in GFP signal in 25 neurons, as reported previously (see Expr817), with one additional cell pair (the DVA neurons) consistently seen in the tail.

Expr16571

In wild-type animals cultured under normal laboratory conditions, we did not observe explicit GFP::NUMR-1 expression. Strikingly, upon nucleolar stress by the inhibition of rRNA transcription using actinomycin D or RNAi knockdown of exos-9 or rpl-14, GFP::NUMR-1 expression was activated, and the protein accumulated in distinct nuclear foci under fluorescence microscopy.

Expr3168

GFP was not detectable under the standard laboratory conditions. The GFP signal was observed solely in a vesicular compartment of coelomocytes of the animals treated with Concanamycin A (CON A) or NH4Cl, agents that increase the pH of the cellular acidic compartment. Immunofluorescence detection of the fusion protein using polyclonal anti-GFP antibody showed a broader and coarsely granular cytoplasmic expression pattern in body wall muscle cells, intestinal cells, and a vesicular compartment of coelomocytes.

Expr15811

Under standard laboratory culture conditions, clec-4p::GFP was constitutively expressed in the intestine of worms throughout all developmental stages. However, expression of clec-4p::GFP seemed to be decreased in L4s and adults, in which a strong GFP signal could only be observed in the first intestinal ring (int1) and the posterior intestine. In addition to its intestinal expression, clec-4p::GFP is constitutively expressed in amphid neurons from the first larval to the adult stage.