Expr10767

The authors confirmed the expression of egl-30 in ADF neurons and the major olfactory sensory neurons AWB and AWC by examining the coexpression of a transcriptional reporter, KP326 (Pegl-30::gfp) (Lackner et al., 1999), with mCherry reporters expressed in these neurons.

Expr12670

egl-8 is expressed in the vas deferens, spicule protractor muscles, diagonal muscles and a male-specific neuron that is probably CP8 or CP9 and is also widely expressed in the nervous system, as expected from previous work (Lackner et al., 1999; Miller et al., 1999).

Expr8555

Approximately 4 h before hatching, the relative abundance of the R subunit begins to increase. When the nematodes hatch the content of R reaches a peak value 6- to 8-fold higher (by densitometry and direct measurement of CAMP binding activities) than the amount observed in embryos. The relative abundance of R observed in newly hatched C. elegans is maintained throughout four larval stages Ll - L4. Changes in R expression were similar in cytosolic and particulate fractions at all stages. As the nematodes become adults and mature into egg-laying animals, an apparent decrease in R content is observed, and proteolytically cleaved 35kDa fragments of R are found in fractions of adults.