Expr13691

LST-1 expression was found in the distal-most cells in the germline, also in the proximal loop region and oocytes in young adult hermaphrodites. Mostly cytoplasmic, enriched in peri-nuclear granules at the distal-most germ cells.

Expr14791

sptl-1::GFP fluorescence was detected in the intestine as well as some unidentified cells of the head from early embryonic stage throughout development. In intestinal cells, SPTL-1::GFP displayed a cytoplasmic punctate, yet clear peri-nuclear, pattern during embryogenesis and developed into a predominantly cytoplasmic punctate pattern in larvae and adults.

Expr14790

sptl-1::GFP fluorescence was detected in the intestine as well as some unidentified cells of the head from early embryonic stage throughout development. In intestinal cells, SPTL-1::GFP displayed a cytoplasmic punctate, yet clear peri-nuclear, pattern during embryogenesis and developed into a predominantly cytoplasmic punctate pattern in larvae and adults.

Expr14789

sptl-1::GFP fluorescence was detected in the intestine as well as some unidentified cells of the head from early embryonic stage throughout development. In intestinal cells, SPTL-1::GFP displayed a cytoplasmic punctate, yet clear peri-nuclear, pattern during embryogenesis and developed into a predominantly cytoplasmic punctate pattern in larvae and adults.

Expr15278

ENPL-1::sfGFP was broadly expressed throughout the animal, with strong expression in neurons, vulva, germline and intestines. To confirm the neuronal localization of ENPL-1, we co-expressed ENPL-1::sfGFP with a pan neural nuclear RFP and showed that ENPL-1::sfGFP is present in a peri-nuclear pattern characteristic of the ER. Furthermore, the expression of ENPL-1 was also observed at the early developmental stages in the embryo.

Expr10052

nhr-111 transcripts were detected by real-time qRT- PCR at modest levels in embryos, but decreased progressively during larval development.The nhr-111::gfp reporter was consistently expressed in at least eight pairs of neurons in the head, the sensory PVD neurons of the posterior lateral body wall, the pharynx, intestine (most often in the posterior- and anterior- most cells), the dorsal peri-vulva region of adults (which may be either uterine or vulval cells), and the somatic gonad precursor cells. Among the head neurons was one prominent pair of sensory neurons just posterior to the nerve ring and at least one pair of neurons or support cells that appear to be inner or outer labial sensory cells. We also observed weak and variable expression in a subset of ventral nerve cord motorneurons. The temporal dynamics of nhr-111::gfp were consistent with nhr-111 qRT-PCR results: expression was very bright in the Z1 and Z4 somatic gonad precursor cells in embryos and early L1, but decreased in the developing gonad at later stages and was relatively faint in other cells.