In L1 hatchlings, expression is dim throughout the animal, but KIN-20 can be observed in the nuclei of neurons. The VD neurons send out growth cones post-embryonically during the L1-L2 molt (Knobel et al., 1999), and during axon extension, expression in the VDs is lower than that in DD neurons.
The transgene oxEx81 expresses GFP from the acr-5 promoter as a chimeric protein with the myristoylation sequence from GAP-43 (Knobel et al. 2001). In these transgenic worms, GFP is predicted to be localized to membranes in a subset of neuron profiles. After staining with anti-GFP antibodies, gold particles accumulate along the plasma membrane in a subset of neurons in the nerve ring.
sem-4 is expressed in all rectal cells in L1s hermaphrodites (i.e., Y, U, B, F, K, and K') and in all rectal cells, including P12.pa, in L3 or older worms.
Pdaf-6GFP was expressed in the amphid sheath glia. Expression was also seen in amphid socket cells, the phasmid sensory organ sheath and socket cells, cells of the excretory system (the excretory canal, duct, pore, and gland cells), the vulval E and F cells, the K, K', F, and U rectal epithelial cells, and less frequently in posterior intestinal cells.