Using a GFP transgenic reporter for pdi-1, we detected GFP in embryonic hypodermis from the comma stage till 3-fold embryos. We did not detect neuronal expression.
We observed cytosolic tagged GCK-2 throughout vulval development (VPCs). We observed the expression of GCK-2 in all tissues, including the germline and embryos in the adult hermaphrodite.
We observed widespread expression of daf-18 genomic GFP, including within the intestine, neurons, and hypodermis. We did not observe expression from our daf-18(+) genomic transgene in the PGCs.
We furthermore note that a pha-4 reporter allele that we generated through CRISPR/Cas9 genome engineering is continuously expressed throughout the entire pharyngeal nervous system, at all postembryonic stages