An HA-tagged ALFA-1 translational reporter was expressed ubiquitously during embryonic development and was enriched in the intestine in later stages of embryonic development.
Consistent with previous findings (Kim and Li, 2004), we found that flp-20 is expressed in the TRNs (ALM, AVM, PLM, PVM), along with a few other neurons (ASE, LUA, and PVC).
Immunofluorescence analysis showed that HA::TagRFP::NRDE-2 was expressed in the nuclei of all/most cells of developing embryos, a result consistent with previous reports on NRDE-2 expression patterns (Guang et al. 2010). We also detected 3xFLAG::GFP::MTR-4 and HA::TagRFP::NRDE-2 expression in the germline. In adult C. elegans pachytene stage germ cells, MTR-4 and NRDE-2 appeared largely colocalized with both proteins concentrated near the nuclear periphery, which is the site of chromatin localization in these cells (Goldstein 1982). Finally, MTR-4, but not NRDE-2, localized to the nuclear interior of germ cells.
Crem-MSS-1::HA expression was first detected in large vesicles and on the plasma membrane of spermatocytes, with intensity increasing and localization restricted to secretory vesicles in mature spermatids. The secretory vesicles of nematode sperm, known as membranous organelles (MOs), fuse with the plasma membrane upon ejaculation and sperm activation.
ODR-2::HA was observed on cell surfaces in the nervous system. The protein was found on the cell bodies of many neurons in the head and tail, on many neuronal processes running along the major (dorsal and ventral) nerve cords, and on the surface of numerous sublateral nerve cords and circumferential commissures.
pbah-1::GFP is expressed in the lateral seam cells, a subset of the hypodermis. Expression was detectable beginning in the early larval stages (L1/L2) and continuing into adulthood, consistent with the temporal expression reported in a functional genomics study. The localization at the protein level was confirmed with a full-length BAH-1 with an added C-terminal HA tag.