Consistent with previous findings (Kim and Li, 2004), we found that flp-20 is expressed in the TRNs (ALM, AVM, PLM, PVM), along with a few other neurons (ASE, LUA, and PVC).
Ce-cdd-2 first appeared in the 8 h sample (late L1), and then was more or less constitutively expressed up to the 34 h sample (young adult), after which expression increased in the 3640 h samples.
Expressed in all stages from eggs (time point 0) to 40 h during worm development. CeRh1 expression was rapidly up-regulated 2 h after fertilization, and this high-level expression was maintained throughout development until the 40-h time point.
Although the cpz-1 transcript is present throughout development, it is least expressed in embryos and during L1 to L2 growth. The level of the cpz-1 transcript increased significantly prior to the L2/L3 molt at 16 h and decreased after the L2/L3 molt (~20 h), and then periodically increased before the L3/L4 molt (~24 h) and the L4/adult molt (~34 h). In addition, a significant increase in its level occurred during the period when the early part of embryogenesis takes place (~40 h after L1). During adulthood there is a 23-h period when the fertilized eggs go through the process of embryogenesis.