Expr16486

This reporter allele allows us to infer spig-2 gene expression in multiple glial cells.

Expr14687

Consistent with previous findings (Kim and Li, 2004), we found that flp-20 is expressed in the TRNs (ALM, AVM, PLM, PVM), along with a few other neurons (ASE, LUA, and PVC).

Expr14718

A transcriptional reporter for arg-1, a Notch ligand gene, is expressed in mDTCs and not in hDTCs or any other regulatory somatic gonad cells, allowing us to distinguish mDTCs from hDTCs.

Expr13409

When the fluorescent reporter Venus was fused to the 3.5 kb lury-1 promoter (lury-1prom), the expression of Venus was observed only in two classes of pharyngeal neurons. These neurons were identified as the M1 and M2 neurons by their morphology, position, and the co-expression of established cell markers (Kim and Li, 2004; Refai et al., 2013). We found that the lury-1prom::Venus reporter was robustly expressed in M1 in all larval stages and in adults, whereas its expression in M2 was not detectable in most L1-L3 worms but started to increase from the L4 stage.

Expr1334

In larvae and adults, lim-4::GFP expression was confined to neurons in the worm's anterior ganglia; embryonic expression was not examined in detail. Expression from both transgenes was observed in the AWB neurons but not in other sensory neurons. lim-4::GFP1 was also expressed in one RME motor neuron (RMEV), two RMD motor neurons (RMDL and RMDR), and the RID, RIV, SAA and SIA interneurons. lim-4::GFP2 was expressed in the same neurons, except for RID and RMEV. Expression in the GABAergic RME motor neurons, which control head movement. In a subsequent study, lim-4 was found to be expressed in SMB neurons, other than SIA neurons (Kim et al., 2015; Expr12499).

Expr9051

In wild-type embryos, ten-1a::gfp is first expressed in a cluster of cells in the anterior half at approximately 150 minutes after fertilization. These cells are precursors to the hypodermal cells, which are evident at 300 minutes post-fertilization, when the cells intercalate and begin the process of ventral closure, and to pharyngeal and intestinal cells, which are evident beginning at the bean stage. In later stages, strong expression of ten-1a::gfp persists in pharyngeal and intestinal cells, and appears in several head neurons. Examination of L1 larvae and adults allowed us to identify 8 pharyngeal cells that express ten-1a::gfp: the three marginal cells mc1, the three marginal cells mc3, and the neurons M2L and M2R. Adults also express ten-1a::gfp in vulva muscles, the gonad distal tip cells, the intestine, several tail neurons including DVB and some other cells.