Under normal osmolarity conditions, AQP-8::GFP localized within the excretory cell soma and throughout the entire anterior excretory canal. Within the posterior excretory canal, the signal intensity decreased from the soma toward the tail region, although the canal is fully extended throughout the whole body in adults (Khan et al., 2013; Kolotuev et al., 2013). In most animals, the signal was only visible along ~80% of the length of the posterior canal.
FHOD-1::GFP is localized in F-actin rich muscles, including BWMs, pharyngeal muscles, and vulval muscles. FHOD-1 is present diffusely in the elongated bands of F-actin rich embryonic myoblasts. FHOD-1 is not detectable in F-actin rich BWMs of L1 larvae but appears as puncta along muscle edges and as striations across the BWM contractile lattice in L2 and L3 larvae.
As reported previously, the promoters of the a, b, and f isoforms drove expression in the whole body except for the pharynx (isoform a), a few tissues (isoform b), and almost all tissues except the gonad (isoform f).We also confirmed Venus expression in head neurons including ASER, by these promoters.