sem-4 is expressed in all rectal cells in L1s hermaphrodites (i.e., Y, U, B, F, K, and K') and in all rectal cells, including P12.pa, in L3 or older worms.
The sec-22p::mCherry::sec-22 transgene showed expression in most, if not all, somatic tissues. This is similar to the expression pattern of a previously reported transcriptional sec-22p::GFP fusion (Hunt-Newbury et al. 2007). It should be noted that the apparent lack of mCherry::SEC-22 expression in the germline is likely due to the strong suppression of multi-copy transgenes in this tissue (Kelly et al. 1997).
NHR-23::GFP somatic cell expression persisted in L4 larvae, specifically in hypodermal cells of the head, vulval precursor cells of hermaphrodites, and hypodermal and tail cells of males. Notably, NHR-23::GFP was also expressed in the sperm-producing germlines of males and L4 hermaphrodites, but not in hermaphrodites producing only oocytes. Previous studies missed this spermatocyte expression as transgenes are frequently silenced in the germline (Kelly et al., 1997) and immunolabeling was limited to embryos (Kostrouchova et al., 1998, 2001).
NHR-23::GFP somatic cell expression persisted in L4 larvae, specifically in hypodermal cells of the head, vulval precursor cells of hermaphrodites, and hypodermal and tail cells of males. Notably, NHR-23::GFP was also expressed in the sperm-producing germlines of males and L4 hermaphrodites, but not in hermaphrodites producing only oocytes. Previous studies missed this spermatocyte expression as transgenes are frequently silenced in the germline (Kelly et al., 1997) and immunolabeling was limited to embryos (Kostrouchova et al., 1998, 2001).