Expr15021

Using live imaging of the GFP-tagged proteins, we observed LIN-29 isoform accumulation. In agreement with immunofluorescence-based analysis (Bettinger et al., 1996), we detected signal in the epidermis and in other regions such as the head, the tail and the vulva. In most sites, patterns of the two LIN-29 isoforms appeared to differ.

Expr14653

The expression pattern of the gfp allele that tags both isoforms of lin-29 (lin-29a and lin-29b), matches the expression pattern reported using an antibody directed against both LIN-29 isoforms (Bettinger et al., 1996; Euling et al., 1999). As previously reported, we observed expression in head neurons and the ventral nerve cord. However, we found that this neuronal expression is almost completely restricted to the male and this male-specific expression can be entirely ascribed to the a-isoform.