Expr13498

GFP::MAI-1 (xmSi32) and MAI-1::mCherry (xmSi31) showed the same tissue localization. GFP::MAI-1 is expressed in specific somatic tissues in the nuclei and in the cytoplasm through specific tissues, including the cuticle, hypodermis, rectum, vulva and neurons. Although the expression pattern did not suggest a mitochondria network and MAI-1 does not carry a mitochondrial transport sequence, we tested whether MAI-1 was expressed in the mitochondria by incubating GFP:: MAI-1 with Mitotracker Red CMXRos and found no co-localization between the mitochondrial probe and GFP::MAI-1. These results demonstrate that MAI-1 is indeed not expressed in mitochondria but in the cytoplasm, as previously suggested Ichikawa et al. Additionally, we observed that GFP::MAI-1 is expressed in the nuclei.