PGL-2 is associated with P granules only during postembryonic development; it is not detectable in embryos. PGL-2 is first detected in L1 larvae, and like its transcript, it is not significantly enriched in the germline in newly hatched L1's. PGL-2 appears to accumulate in P granules, with weak signal still remaining in somatic tissues. It remains associated with P granules in adult gonads in both sexes until it disappears during spermatogenesis and during the final stages of oogenesis.
APX-1 protein is detected first in the late 2-cell stage at the anterior periphery of P1. It is found in and EMS at the 4-cell stage. Later, it is seen faintly in P3.
ECT-2::GFP localizes throughout the germline from the mitotically dividing nuclei at the premeiotic tip, where it is enriched at the germ cell membrane, to the end of diakinesis, where it exhibits a stronger nuclear signal.
The steady-state level of mog-1 mRNA varied during development. It peaks in L4 just before the germ line switches from spermatogenesis to oogenesis at the molt from L4 to adult, and it continues in the adult and embryo.
The tail region of male animals injected with CeDOP2-YFP and cat-2-NLS-CFP were also examined. The fluorescence of CFP in adult male ray neurons was very strong and it was impossible to separate it from the fluorescence of YFP.