Expression of ARRD-15 in the body wall muscle was detected by observing localization of endogenous
sup-13/arrd-15 that was C-terminally tagged with GFP (ARRD-15::GFP) by CRISPR/ Cas9-mediated gene editing. The fluorescence levels of ARRD-15::GFP were strongest in the nerve ring and also detected at low levels in the body wall muscle and localized in the diffuse cytoplasm. This expression pattern is consistent with the mRNA distribution data in the GExplore database (Hutter et al., 2009).