sem-4 is expressed in all rectal cells in L1s hermaphrodites (i.e., Y, U, B, F, K, and K') and in all rectal cells, including P12.pa, in L3 or older worms.
NAB-1::GFP expression is restricted to epithelia and neurons. The earliest expression was observed in the hypodermis of 2-fold-stage early embryos. Immediately prior to hatching, this expression became restricted to the epithelial excretory canal and the nervous system, including the central nervous system and the motoneurons (dorsal and ventral nerve cords. In L3 and L4 larvae, NAB-1::GFP also localized transiently at the membranes of the developing vulva epithelia. Also expressed in distal tip cell (pers. comm. from Wesley Hung 11-17-07.)
Pdaf-6GFP was expressed in the amphid sheath glia. Expression was also seen in amphid socket cells, the phasmid sensory organ sheath and socket cells, cells of the excretory system (the excretory canal, duct, pore, and gland cells), the vulval E and F cells, the K, K', F, and U rectal epithelial cells, and less frequently in posterior intestinal cells.
About 20% GABAergic presynaptic terminals (RFP puncta) co-localized with postsynaptic NKB-1 (GFP) localization (103 co-localization with GFP puncta in totally observed 470 RFP puncta (n = 20 animals)). The majority of the postsynaptic Na+/K+ ATPase localization did not overlap with that of GABAergic synapses, suggesting a predominant localization of the Na+/K+ ATPase at cholinergic postsynaptic sites.