GFP distribution looks like the one described for pes-1 in Hope, I.A., ('94) Development, 120, 505-14 but expression driven by this construct is of a higher intensity than when the reporter was inserted before the pes-1 stop codon (see fUL#JW5).
Diffuse mosaic expression is seen in the pharynx from the L2 larval stage to adult. This expression is possibly spurious (Hope, I.A. (1991). Development, 113, 399. Krause, M. et al. (1994). Dev. Biol., 166, 133.). However, the upstream region used in this construct is 5.7kb and should contain all upstream promoter elements.
In the meiotic cells of the gonad including oocytes and in the interphase cells of 200-cell embryos, DNA topoisomerase I was localized in nuclei. At the comma stage, a more narrowed concentration of DNA topoisomerase I was observed in the nucleoli of gut cells.