6 Expression patterns found (0.009 seconds)

In early larvae, this reporter was expressed in a cluster of posterior cells including rectal epithelial cells, the overlying dorsal and ventral body wall muscles, the stomatointestinal muscles, the anal depressor muscle, and P11/12, along with weak expression in several head neurons, which is largely consistent with smFISH data on egl-20 transcript localization (Harterink et al., 2011a) and previous transgenes (Whangbo and Kenyon, 1999). This reporter also labeled several posterior neurons and their projections along the ventral nerve cord that terminated in the nerve ring. Tagged Wnt protein clearly localized near reporter-labeled axons in the head.

Using transcriptional reporters of the five Wnt ligands [cis-regulatory elements of the Wnt ligands placed upstream of a nuclear GFP (Gleason et al., 2006)], we observed that three Wnt ligands (CWN-1, CWN-2 and MOM-2) are expressed in the embryo at the time of the terminal division of the SMDD/AIY mother cell. We could not detect expression of the two remaining Wnt ligands (LIN-44 and EGL-20) at that time but start seeing expression at later stages, during elongation, in the posterior end of the embryo. Interestingly, at the time of the terminal division of the SMDD/AIY mother cell, cwn-1, cwn-2 and mom-2 are transcribed at a higher level in the posterior region of the embryo than in the anterior region. These observations are consistent with an analysis of the transcription pattern of Wnt ligands by fluorescent in situ hybridization (Harterink et al., 2011). cwn-1, cwn-2 and mom-2 are transcribed in several tissues: cwn-1 (posterior muscle), cwn-2 ( posterior neuronal progenitors, posterior epidermis, intestine and posterior muscle) and mom-2 (posterior epidermis and muscle). Their zygotic expression starts during gastrulation and remains during embryonic elongation.

Using transcriptional reporters of the five Wnt ligands [cis-regulatory elements of the Wnt ligands placed upstream of a nuclear GFP (Gleason et al., 2006)], we observed that three Wnt ligands (CWN-1, CWN-2 and MOM-2) are expressed in the embryo at the time of the terminal division of the SMDD/AIY mother cell. We could not detect expression of the two remaining Wnt ligands (LIN-44 and EGL-20) at that time but start seeing expression at later stages, during elongation, in the posterior end of the embryo. Interestingly, at the time of the terminal division of the SMDD/AIY mother cell, cwn-1, cwn-2 and mom-2 are transcribed at a higher level in the posterior region of the embryo than in the anterior region. These observations are consistent with an analysis of the transcription pattern of Wnt ligands by fluorescent in situ hybridization (Harterink et al., 2011). cwn-1, cwn-2 and mom-2 are transcribed in several tissues: cwn-1 (posterior muscle), cwn-2 ( posterior neuronal progenitors, posterior epidermis, intestine and posterior muscle) and mom-2 (posterior epidermis and muscle). Their zygotic expression starts during gastrulation and remains during embryonic elongation.

Using transcriptional reporters of the five Wnt ligands [cis-regulatory elements of the Wnt ligands placed upstream of a nuclear GFP (Gleason et al., 2006)], we observed that three Wnt ligands (CWN-1, CWN-2 and MOM-2) are expressed in the embryo at the time of the terminal division of the SMDD/AIY mother cell. We could not detect expression of the two remaining Wnt ligands (LIN-44 and EGL-20) at that time but start seeing expression at later stages, during elongation, in the posterior end of the embryo. Interestingly, at the time of the terminal division of the SMDD/AIY mother cell, cwn-1, cwn-2 and mom-2 are transcribed at a higher level in the posterior region of the embryo than in the anterior region. These observations are consistent with an analysis of the transcription pattern of Wnt ligands by fluorescent in situ hybridization (Harterink et al., 2011). cwn-1, cwn-2 and mom-2 are transcribed in several tissues: cwn-1 (posterior muscle), cwn-2 ( posterior neuronal progenitors, posterior epidermis, intestine and posterior muscle) and mom-2 (posterior epidermis and muscle). Their zygotic expression starts during gastrulation and remains during embryonic elongation.

Using transcriptional reporters of the five Wnt ligands [cis-regulatory elements of the Wnt ligands placed upstream of a nuclear GFP (Gleason et al., 2006)], we observed that three Wnt ligands (CWN-1, CWN-2 and MOM-2) are expressed in the embryo at the time of the terminal division of the SMDD/AIY mother cell. We could not detect expression of the two remaining Wnt ligands (LIN-44 and EGL-20) at that time but start seeing expression at later stages, during elongation, in the posterior end of the embryo. Interestingly, at the time of the terminal division of the SMDD/AIY mother cell, cwn-1, cwn-2 and mom-2 are transcribed at a higher level in the posterior region of the embryo than in the anterior region. These observations are consistent with an analysis of the transcription pattern of Wnt ligands by fluorescent in situ hybridization (Harterink et al., 2011). cwn-1, cwn-2 and mom-2 are transcribed in several tissues: cwn-1 (posterior muscle), cwn-2 ( posterior neuronal progenitors, posterior epidermis, intestine and posterior muscle) and mom-2 (posterior epidermis and muscle). Their zygotic expression starts during gastrulation and remains during embryonic elongation.

Using transcriptional reporters of the five Wnt ligands [cis-regulatory elements of the Wnt ligands placed upstream of a nuclear GFP (Gleason et al., 2006)], we observed that three Wnt ligands (CWN-1, CWN-2 and MOM-2) are expressed in the embryo at the time of the terminal division of the SMDD/AIY mother cell. We could not detect expression of the two remaining Wnt ligands (LIN-44 and EGL-20) at that time but start seeing expression at later stages, during elongation, in the posterior end of the embryo. Interestingly, at the time of the terminal division of the SMDD/AIY mother cell, cwn-1, cwn-2 and mom-2 are transcribed at a higher level in the posterior region of the embryo than in the anterior region. These observations are consistent with an analysis of the transcription pattern of Wnt ligands by fluorescent in situ hybridization (Harterink et al., 2011). cwn-1, cwn-2 and mom-2 are transcribed in several tissues: cwn-1 (posterior muscle), cwn-2 ( posterior neuronal progenitors, posterior epidermis, intestine and posterior muscle) and mom-2 (posterior epidermis and muscle). Their zygotic expression starts during gastrulation and remains during embryonic elongation.