Expr13289

prkl-1 is expressed in VNC neurons. In contrast to Prickle localization in other CE models (Jiang et al., 2005; Narimatsu et al., 2009; Yin et al., 2008), PRKL-1 (n = 6) was not observed to exhibit recognizable membrane or polarized subcellular localization. Relatively uniform PRKL-1 cytoplasmic localization was observed in VNC neurons.

Expr15953

mNG::mls-2 knock-in animals displayed wild-type AWC asymmetry as determined by the expression of the AWCON marker str-2p::TagRFP, suggesting that mNG::MLS-2 fusion protein is functional in AWC development. Like GFP::MLS-2 expressed from transgenes, mNG::MLS-2 knock-in was localized in the nucleus of AWC precursor cells in early embryos but was not observed in AWC cells in late embryos or early-stage larvae. Similar to MLS-2 antibody staining and GFP::MLS-2 transgenes (Jiang et al., 2005), mNG::MLS-2 knock-in was localized to the nucleus of a subset of head cells and the M mesoblast in first-stage larvae and adults.

Expr7837

These Psnf-11::GFP fusions are expressed in the same neurons (RMEs, AVL, DVB, RIS, and RID) that stained with the anti-SNF-11 polyclonal antibodies. Expression was also noted in two additional neurons near the pharynx as well as two neurons in the retrovesicular ganglion. There were no apparent differences in expression between the two reporters, suggesting that the 1.9-kb region is sufficient to drive expression in all snf-11 positive cells. In contrast to observations reported previously (Jiang et al., 2005 blue right-pointing triangle), authors did not observe snf-11 expression in the ventral cord inhibitory (DD and VD) motor neurons. However, they did observe robust expression in the body wall, anal, and uterine muscles that was not noted previously. In young animals, expression of the Psnf-11::GFP reporter in muscle cells is the most prominent aspect of the expression pattern.