Expr13282

The knockin strain shows significant Msx/vab-15 expression in embryonic AB.p(lr)apapaa, which is the mother cell of Q and V5 neuroblasts. After the division of this mother cell, Msx/vab-15 expression turns off in V5 neuroblasts but is maintained in Q cells until they divide. P-neuroblast expression of Msx/vab-15 starts before hatching, is maintained while P nuclei migrate into the ventral midline in early L1-stage larvae, and shuts down after P neuroblasts divide.

Expr13464

ETR-1 is expressed in both the germline and the somatic gonadal tissue of the hermaphrodite gonad. A medial optical section through the hermaphrodite gonad shows that ETR-1 exhibits a diffuse, cytoplasmic staining throughout the entire hermaphrodite gonad. We also observed cytoplasmic expression of ETR-1 within both the female and male gametes (egg and sperm, respectively). A superficial optical section of the proximal hermaphrodite gonad shows that ETR-1 is localized specifically to the gonadal sheath cell myofilaments. This result was confirmed by colocalization with an antibody against myosin heavy chain A (MHCA) (Hubbard and Greenstein, 2000; Rose et al., 1997). Further confirmation of ETR-1 being expressed in the somatic gonadal cells emerges when imaging the surface of the distal gonad. ETR-1 exhibits a honeycomb pattern in the distal germ line that is consistent with the pattern observed by Hall et al. (1999) in their description of the individual somatic gonadal sheath cells via TEM and immunofluorescence studies (Hall et al., 1999). Analysis of embryos indicates early embryonic expression of ETR-1 that persists through the comma stage of embryonic development. In early embryos (1- to 16-cell stage), ETR-1 is diffusely cytoplasmic. In older embryos (>32-cell), while there still remains some diffuse cytoplasmic ETR-1 staining, a majority of the ETR-1 is now nuclear.

Expr14580

In adult males, the SWM-1::mCherry protein was present, as expected, in cells in which we observed the transcriptional reporter [Expr14579]: vas deferens cuboidal cells, diagonal muscles and body wall muscles. Within cuboidal cells, SWM-1::mCherry was contained within large vesicles that were closely associated with the apical membrane, which are not well-characterized but have been shown to contain seminal fluid (Smith and Stanfield, 2011; Lints and Hall, 2009; Kimble and Hirsh, 1979). In muscle cells, we observed SWM-1::mCherry at low levels that appeared to be concentrated near the cell cortex. Interestingly, in jn62[swm-1::mCherry], SWM-1::mCherry was also visible in anterior body wall muscles, rather than being restricted to the posterior region, corresponding to slightly higher levels of rescue and protein expression as compared with the Pswm-1 MosSCI reporter strain.