- Expr14721
HA::ALG- 2 was predominantly expressed in embryos but was present also throughout development.
- Expr14719
HA::ALG-5 expression was highest during late stages of larval development (L2, L3, L4) and into adulthood.
- Expr14720
HA::ALG-1 was abundant throughout development, consistent with a central role for ALG-1 in the miRNA pathway.
- Expr14644
TRA-2:HA is detected in larval and adult hermaphrodites, but not in males, consistent with their greatly reduced transcript level.
- Expr13730
An HA-tagged ALFA-1 translational reporter was expressed ubiquitously during embryonic development and was enriched in the intestine in later stages of embryonic development.
- Expr15849
Fluorescence from GFP::EIF-3.G was observed in all somatic cells.
- Expr15850
Fluorescence from GFP::EIF-3.G was observed in all somatic cells.
- Expr15666
Immunofluorescence analysis showed that HA::TagRFP::NRDE-2 was expressed in the nuclei of all/most cells of developing embryos, a result consistent with previous reports on NRDE-2 expression patterns (Guang et al. 2010). We also detected 3xFLAG::GFP::MTR-4 and HA::TagRFP::NRDE-2 expression in the germline. In adult C. elegans pachytene stage germ cells, MTR-4 and NRDE-2 appeared largely colocalized with both proteins concentrated near the nuclear periphery, which is the site of chromatin localization in these cells (Goldstein 1982). Finally, MTR-4, but not NRDE-2, localized to the nuclear interior of germ cells.
- Expr13699
Crem-MSS-1::HA expression was first detected in large vesicles and on the plasma membrane of spermatocytes, with intensity increasing and localization restricted to secretory vesicles in mature spermatids. The secretory vesicles of nematode sperm, known as membranous organelles (MOs), fuse with the plasma membrane upon ejaculation and sperm activation.
- Expr4348
ODR-2::HA was observed on cell surfaces in the nervous system. The protein was found on the cell bodies of many neurons in the head and tail, on many neuronal processes running along the major (dorsal and ventral) nerve cords, and on the surface of numerous sublateral nerve cords and circumferential commissures.