Consistent with previous reports in zygotes (Griffin et al., 2011; Guo and Kemphues, 1995), PAR-1::GFP was enriched on the posterior cortex, in a weak gradient in the posterior cytoplasm, on centrosomes and weakly on P granules. Asymmetric segregation was repeated in each P blastomere, with PAR-1::GFP enriched on the cortex destined for the next P cell daughter. Enrichment of PAR-1 in the germ lineage persisted through gastrulation. Cortical PAR-1 could be detected in most, and perhaps all, embryonic cells, but was present at higher levels in the primordial germ cells Z2 and Z3, daughters of the P4 blastomere.
This construct was expressed faintly in QL and QR, more strongly in the neighboring epidermal cells (dorsal hyp7 cells, ventral P cells and lateral V cells), and in dorsal and ventral body muscle cells.
Staining was observed in all larval and adult stages, localizing to the nuclei of the hypodermal cells, specifically the head (hyp3, hyp4, hyp6, and hyp7) and seam/body (hyp7, H, V, and P) hypodermal cells.