Expr15666

Immunofluorescence analysis showed that HA::TagRFP::NRDE-2 was expressed in the nuclei of all/most cells of developing embryos, a result consistent with previous reports on NRDE-2 expression patterns (Guang et al. 2010). We also detected 3xFLAG::GFP::MTR-4 and HA::TagRFP::NRDE-2 expression in the germline. In adult C. elegans pachytene stage germ cells, MTR-4 and NRDE-2 appeared largely colocalized with both proteins concentrated near the nuclear periphery, which is the site of chromatin localization in these cells (Goldstein 1982). Finally, MTR-4, but not NRDE-2, localized to the nuclear interior of germ cells.

Expr15665

MTR-4 was expressed diffusely within nuclei of all embryonic cells as well as most/all somatic cells in larval animals. The data show that MTR-4 is a ubiquitously or near-ubiquitously expressed, nuclear-localized protein. We also detected 3xFLAG::GFP::MTR-4 and HA::TagRFP::NRDE-2 expression in the germline. In adult C. elegans pachytene stage germ cells, MTR-4 and NRDE-2 appeared largely colocalized with both proteins concentrated near the nuclear periphery, which is the site of chromatin localization in these cells (Goldstein 1982). Finally, MTR-4, but not NRDE-2, localized to the nuclear interior of germ cells.