Expr1920

Expressed in nine DA, 11 AS, ALA, RID, two PDE, two HSN, M5, four vulval muscles 1, four vulval muscles 2, four uterine muscles 1, four uterine muscles 2, two intestine muscles, sphincter muscle, 15 diagonal muscles, two spicule retractors, bodywall muscles, two AM socket cells, two PH socket cells, two distal tip cells.

Expr15666

Immunofluorescence analysis showed that HA::TagRFP::NRDE-2 was expressed in the nuclei of all/most cells of developing embryos, a result consistent with previous reports on NRDE-2 expression patterns (Guang et al. 2010). We also detected 3xFLAG::GFP::MTR-4 and HA::TagRFP::NRDE-2 expression in the germline. In adult C. elegans pachytene stage germ cells, MTR-4 and NRDE-2 appeared largely colocalized with both proteins concentrated near the nuclear periphery, which is the site of chromatin localization in these cells (Goldstein 1982). Finally, MTR-4, but not NRDE-2, localized to the nuclear interior of germ cells.

Expr15665

MTR-4 was expressed diffusely within nuclei of all embryonic cells as well as most/all somatic cells in larval animals. The data show that MTR-4 is a ubiquitously or near-ubiquitously expressed, nuclear-localized protein. We also detected 3xFLAG::GFP::MTR-4 and HA::TagRFP::NRDE-2 expression in the germline. In adult C. elegans pachytene stage germ cells, MTR-4 and NRDE-2 appeared largely colocalized with both proteins concentrated near the nuclear periphery, which is the site of chromatin localization in these cells (Goldstein 1982). Finally, MTR-4, but not NRDE-2, localized to the nuclear interior of germ cells.