Endogenous DVC1 was predominantly expressed during S and G2 phases of the cell cycle. Upon exit from mitosis, DVC1 expression was rapidly downregulated with kinetics similar to that of known APC-Cdh1 targets such as cyclin A, and reaccumulated as cells entered S phase.
Inspection of three independent che-12 pro::GFP transgenic lines showed that che-12 is expressed in only the subset of amphid neurons that lack wing- or finger-like ciliary extensions and in the two phasmid neurons.
To determine in which cell(s) tiar-2 functions, we constructed a GFP-TIAR-2 mini-gene translational reporter and observed broad expression in many cell types, including mechanosensory neurons.
GFP::OIG-8 is exclusively expressed in the olfactory amphid wing (AW)-type sensory neurons AWA, AWB, and AWC, the thermosensory amphid finger neurons (AFD), the gas-sensing BAG neurons, and the harsh touch sensory neurons FLP and PVD.
HSP-4::GFP was exclusively localized in the PVD soma, colocalizing with a rough ER marker TRAM, HSP-4's endoplasmic reticulum localization pattern is consistent with the observation that its mammalian homolog BiP is localized in rough endoplasmic reticulum.