nfyb-1p::gfp transgenic animals showed strong gfp expression in all six IL1s, supporting that the NF-Y complex acts in IL1s to regulate IL1 marker expression.
Apart from the gonads and developing embryos, the CeNF-Y mRNAs were detected in other tissue from C. elegans. In 6-day-old worms, CeNF-YB and CeNF-YC, but not CeNF-YA1 nor CeNF-YA2, were expressed in the cytoplasm of the secretory cells located in the pharyngeal terminal bulb wall, in the neuron cell bodies of the small ganglia surrounding the pharynx and in the neurons running anteriorly to the sensory organs in the animal's head. NF-YC was also found in the excretory cell. Subunits were not expressed in any other organs, such as the intestine, the hypodermis and body wall muscle surrounding the pseudocoelomic space.