Quantitative RT-PCR (qRT-PCR) reactions were also performed on wild type,
rha-1(
tm329), and
glp-4(
bn2ts) worms. The latter strain develops with a very small, underproliferated gonad at the restrictive temperature of 25 centigrades.
rha-1 expression is strongly enriched in the germline since little
rha-1 mRNA was detected in
glp-4 worms synchronized with the L4-young adult wild-type worms. The qRT-PCR data also show that
rha-1 transcript levels are highest in L1 and L4-young adult worms.