Expr2892

Although the cpz-1 transcript is present throughout development, it is least expressed in embryos and during L1 to L2 growth. The level of the cpz-1 transcript increased significantly prior to the L2/L3 molt at 16 h and decreased after the L2/L3 molt (~20 h), and then periodically increased before the L3/L4 molt (~24 h) and the L4/adult molt (~34 h). In addition, a significant increase in its level occurred during the period when the early part of embryogenesis takes place (~40 h after L1). During adulthood there is a 23-h period when the fertilized eggs go through the process of embryogenesis.

Expr1914

Ce-cdd-2 first appeared in the 8 h sample (late L1), and then was more or less constitutively expressed up to the 34 h sample (young adult), after which expression increased in the 3640 h samples.

Expr3969

Expressed in all stages from eggs (time point 0) to 40 h during worm development. CeRh1 expression was rapidly up-regulated 2 h after fertilization, and this high-level expression was maintained throughout development until the 40-h time point.

Expr11801

Using semi-quantitative RT-PCR, we show that C54G7.3 is somatically expressed. C54G7.3 is first detected at 24 h following hatching, when grown at 25 C. C54G7.3 continued to be expressed at 48 h and 72 h. Adults first appeared at the 72 h time point and C54G7.3 showed clear expression despite the significant attenuation of the germline.C54G7.3 and chs-2 expression declined following onset of adulthood (72 h). C54G7.3 was expressed in the absence of a germline and the expression declined once all the worms reached adulthood. Only one of the two predicted transcripts of C54G7.3 includes sequence coding for the PDA domain.

Expr13345

Isoforms (A, B, F, G, H, and I) share the same initiator codon, and this promoter reporter is expressed in body wall and vulva muscles.

Expr891

equivalent levels of the predicted 52 kDa LAP-1 protein from 10 to 40 hrs and a LAP-1 band is seen from 2 to 8 h, albeit at a lower level.

Expr688

let-23 expression is first observed in the early L3 larvae (1-3 h after L2 molt, 1.5-3.5 h before division of the Pn.p cells), in all six Pn.p cells and is concentrated in a lateral ring. let-23 appears to be specifically associated with the cell junction. Let-23 expression decreases rapidly in the 2o cells (P5.p and P7.p) starting at 3 h and disappearing completely by 3.5 h after L2 molt. Expression in P3.p, P4.p and P8.p decreases gradually until no longer seen 3.5-4 h after L2 molt. Expression in P6.p increases starting at 4-4.5 h after L2 molt. At this stage expression is observed in a lateral ring in the plasma membrane. At approximately 4-4.5 h after the L2 molt, expression is localized to the P6.p cell junction in 69% of the animals and staining is more punctate. In the remaining 31% of animals staining is broadly distributed on the apical plasma membrane of P6.p.

Expr4703

Expressed in body wall, pharyngeal and vulva muscle, H cell.

Expr1833

The level of lin-28 mRNA was constant over the entire period examined, from the start of the L1 (6 h) to the early- to mid-L3 (36 h).

Expr4394

Antibody staining was detected in the H-shape excretory cells and the intestine.