Expr14934

Expression of the extrachromosomal act-3p::rfp transgene was only observed in the pharynx in wild-type animals, consistent with previous studies (Hunt-Newbury et al., 2007).

Expr13362

The sec-22p::mCherry::sec-22 transgene showed expression in most, if not all, somatic tissues. This is similar to the expression pattern of a previously reported transcriptional sec-22p::GFP fusion (Hunt-Newbury et al. 2007). It should be noted that the apparent lack of mCherry::SEC-22 expression in the germline is likely due to the strong suppression of multi-copy transgenes in this tissue (Kelly et al. 1997).

Expr10536

Pklp-4::GFP is expressed in the pharynx, the intestine and throughout the nervous system including the nerve ring, and the lateral, dorsal and ventral nerve cords. This klp-4 expression pattern is consistent with previous expression studies (McKay et al., 2003; Hunt-Newbury et al., 2007). In addition, Pklp-4::GFP is expressed in many head, tail and ventral cord neurons, including glr-1-expressing neurons based on the overlapping expression pattern of Pklp-4::GFP and a glr-1 transcriptional reporter (Pglr-1::dsRED).

Expr10160

In larvae and adult hermaphrodites, the F40F11.2/mig-38 promoter-driven GFP signal is found mainly in neurons, muscles, and other contractile tissues such as the spermatheca (Hunt-Newbury et al., 2007). mig-38 expression was additionally detected in the somatic gonad precursors, Z1 and Z4 and later within DTCs during larval and adult stages. Gonadal dissections allowed detection of F40F11.2p::GFP expression within the gonadal sheath cells. However, expression was not observed in the germ cells or their precursors Z2 and Z3.