Expr9018

Both let-381::gfp and anti-LET-381 immunostaining showed similar let-381 expression patterns, which begin during embryogenesis and persist in a small number of cells throughout larval development. As expected of a transcription factor, LET-381 localizes to the nucleus. Double-labeling experiments using the let-381::gfp fusion or anti-LET-381 with anti-FOZI-1 staining (to label M lineage cells) showed that LET-381 is transiently expressed in the M lineage and is first detected in M.dlp and M.drp as they begin to divide. Expression continued in both daughters of these divisions [M.d(l/r)pa and M.d(l/r)pp] until the cells differentiated.

Expr8279

FOZI-1 expression in the M lineage was first detectable in the nuclei of M.d and M.v at the 2-M stage (in 43% of the animals stained, n=21). This expression persisted through the next three rounds of cell divisions, such that all animals (n>50) at the 4-M through to the 16-M stage showed FOZI-1 expression in the M lineage. At the 16-M stage, two cells on the ventral side (M.vlpa and M.vrpa) divide one more time to produce two SMs and two BWM precursors and reach the 18-M stage. At the 18-M stage, FOZI-1 was still detectable in the undifferentiated BWMs and CCs, but not in the SMs (n=22). This expression of FOZI-1 at the 18-M stage appeared transiently and quickly became undetectable in the differentiated BWMs and CCs. FOZI-1 expression was not detected in the SM lineage.

Expr689

let-60 ras::lacZ. The Vulval lineage: First detected in L3 larvae (before vulval induction). Faint staining observed in P3.p-P8.p. Staining becomes stronger as VPCs begin dividing and fusion protein is expressed through adulthood. Faint staining observed in hyp7. Strong staining in vulA, vulB, vulC, vulD, vulE and vulF. Myoblast lineage: L1 (shortly after division of M) - Staining detected in M.d and M.v. Late L1, faint staining in progeny of M.v (body muscle) including SM (progeny of M.d (body muscle) ceases staining). L3: 8 progeny of SM (vulval muscle) stain before and after differentiation in muscle cells. Gonadal lineage: At hatching Z1 and Z4 gonadal cells stain. Progeny Z1 and Z4 that form distal tip cells (dtcs) and dtcs stain throughout larval development-adulthood. L4: Anchor cells (ac) of somatic gonad stains transiently at apex of invaginating vulva and continues until late L4 when ac nucleus fuses with uterine tissue. L4-adult expression (but not lacZ) Intense gfp near germline nuclei along border of distal arm of the gonad and in some places extended into the rachis. Muscle: L1-adult: All body wall muscle cells stain. Pharyngeal muscle pmp3-8 begin staining in L1 and continue until adulthood. Hypodermis: Begin staining in L2-3 larvae but consistent staining does not occur until the L4 stage and continues until adulthood. Hypodermal cells staining include V and H lineage-derived seam cells and V and H derived lateral hypodermal cells. Ventral hypodermal cells derived from P lineage also stain weakly but consistently in the adult. Intestine: Intestinal cells of late L1 larvae stain briefly during their terminal division. No staining after L2. Nervous system and excretory cells: extensive staining but not entirely at hatching throughout development. Beginning L1 - adulthood: Many ventral cord neurons stain positively identified include FLPL,R AVKL,R and either AIMR or AIYR based on co-staining with an anti-FMRF amide and an anti-galactosidase antibody. Nucleus of excretory cell stains in L1 to adulthood.