The expression of
pat-4/ILK was examined specifically in AFD and AIY neurons by using a transcriptional
pat-4p::gfp reporter construct (Dupuy et al. 2007) expressed in combination with gcy- 8p::mCherry or
ttx-3p::rfp constructs. Surprisingly,
pat-4p::gfp was neither expressed in AFD nor in AIY neurons, but was present in adjacent cells, which may be ADF and/or AIM neurons (chemosensory/oxygencsensory neurons and interneurons, respectively). In addition expression was observed in body-wall muscle and in the gonad, as previously described (Mackinnon et al. 2002).